TFAM antibody (AA 75-124)

Catalog No. ABIN6736760

This anti-TFAM antibody is a Rabbit Polyclonal antibody detecting TFAM in WB, IHC and IHC (p). Suitable for Human, Mouse, Rabbit, Dog and Guinea Pig.

Quick Overview for TFAM antibody (AA 75-124) (ABIN6736760)

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivity: Human, Mouse, Rabbit, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TFAM Antibody

Binding Specificity: AA 75-124

Brand: IHC-plus™

Specificity: Human TFAM

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig (91%) Dog, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa75-124 of human TFAM (Q00059, NP_003192). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon (100%), Monkey, Panda, Bovine, Horse, Pig (91%), Galago, Dog, Bat, Rabbit (83%), Mouse (75%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: MtTFA / TFAM

Background: Name/Gene ID: TFAM
Family: Transcription factor

Synonyms: TFAM, MTTF1, TCF-6, TCF6, TCF6L1, Transcription factor 6-like 2, Transcription factor 6-like 3, TCF6L2, TCF6L3, Transcription factor 6, Transcription factor 6-like 1, MTTFA

Gene ID: 7019

NCBI Accession: NP_003192

UniProt: Q00059

Pathways: Chromatin Binding