MED21 antibody (AA 74-123)

Catalog No. ABIN6737805

This anti-MED21 antibody is a Rabbit Polyclonal antibody detecting MED21 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Pig, Chicken, Hamster, Monkey, Rabbit and Xenopus laevis.

Quick Overview for MED21 antibody (AA 74-123) (ABIN6737805)

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Pig, Chicken, Hamster, Monkey, Rabbit, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED21 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED21 Antibody

Binding Specificity: AA 74-123

Specificity: Human MED21 / SURB7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa74-123 of human MED21 (Q13503, NP_004255). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus, Trout, Salmon, Smelt, Zebrafish (100%), Opossum, Platypus, Lizard (92%), Stickleback (91%), Panda (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Alternative Name: MED21

Background: Name/Gene ID: MED21

Synonyms: MED21, HSrb7, RNAPII complex component SRB7, SRB7, SURB7, Mediator complex subunit 21

Gene ID: 9412

NCBI Accession: NP_004255

UniProt: Q13503

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha