POLR2I antibody (N-Term)

Catalog No. ABIN6737851

This Rabbit Polyclonal antibody specifically detects POLR2I in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig, Monkey, Zebrafish (Danio rerio), Bat, Drosophila melanogaster and Xenopus laevis.

Quick Overview for POLR2I antibody (N-Term) (ABIN6737851)

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig, Monkey, Zebrafish (Danio rerio), Bat, Drosophila melanogaster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2I Antibody

Binding Specificity: N-Term

Specificity: Human POLR2I

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human POLR2I (P36954, NP_006224). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Xenopus, Trout, Catfish, Stickleback, Zebrafish, Drosophila, Beetle (100%), Turkey (92%), Arabidopsis (91%), Slime mold, Rice, Grape (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR2I

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Alternative Name: POLR2I

Background: Name/Gene ID: POLR2I

Synonyms: POLR2I, HRPB14.5, RNA polymerase II subunit B9, RPB14.5, RPB9

Gene ID: 5438

NCBI Accession: NP_006224

UniProt: P36954

Pathways: Regulatory RNA Pathways