SF3A1 antibody (AA 121-170)

Catalog No. ABIN6737939

The Rabbit Polyclonal anti-SF3A1 antibody has been validated for WB. It is suitable to detect SF3A1 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Xenopus laevis, Pig, Bat, Hamster, Monkey and Drosophila melanogaster.

Quick Overview for SF3A1 antibody (AA 121-170) (ABIN6737939)

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Xenopus laevis, Pig, Bat, Hamster, Monkey, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SF3A1 Antibody

Binding Specificity: AA 121-170

Specificity: Human SF3A1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa121-170 of human SF3A1 (Q15459, NP_005868). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus, Drosophila (100%), Chicken (92%), Beetle (90%), Salmon, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Alternative Name: SF3A1 / SF3A120

Background: Name/Gene ID: SF3A1

Synonyms: SF3A1, Pre-mRNA processing 21, PRPF21, SAP 114, SAP114, SF3A120, Splicing factor 3 subunit 1, Splicing factor 3A subunit 1, PRP21

Gene ID: 10291

NCBI Accession: NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization