EWSR1 antibody (AA 173-222)

Catalog No. ABIN6737947

The Rabbit Polyclonal anti-EWSR1 antibody has been validated for WB. It is suitable to detect EWSR1 in samples from Human, Mouse, Rat, Cow, Pig, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Monkey and Bat.

Quick Overview for EWSR1 antibody (AA 173-222) (ABIN6737947)

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EWSR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EWSR1 Antibody

Binding Specificity: AA 173-222

Specificity: Human EWSR1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Pig, Guinea pig (100%) Chicken (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa173-222 of human EWSR1 (Q01844). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Platypus (92%), Turkey, Zebra finch, Chicken (84%), Xenopus, Stickleback (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EWSR1

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Alternative Name: EWSR1 / EWS

Background: Name/Gene ID: EWSR1

Synonyms: EWSR1, EWS, RNA-binding protein EWS, BK984G1.4, EWS oncogene

Gene ID: 2130

UniProt: Q01844