Perforin 1 antibody (AA 146-195)

Catalog No. ABIN6738024

The Rabbit Polyclonal anti-Perforin 1 antibody has been validated for WB. It is suitable to detect Perforin 1 in samples from Human, Cow, Pig and Horse.

Quick Overview for Perforin 1 antibody (AA 146-195) (ABIN6738024)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human, Cow, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 146-195

Specificity: Human PRF1 / Perforin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (92%) Horse, Pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of human PRF1 (P14222, NP_005032). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Dog, Bat, Rabbit (92%), Cat, Horse, Pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: PRF1 / Perforin

Background: Name/Gene ID: PRF1

Synonyms: PRF1, HPLH2, Perforin, p1, Perforin1, PFP, Cytolysin, FLH2, Perforin-1

Gene ID: 5551

NCBI Accession: NP_005032

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis