MARCH5 antibody (C-Term)

Catalog No. ABIN6738065

The Rabbit Polyclonal anti-MARCH5 antibody has been validated for WB. It is suitable to detect MARCH5 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Chicken, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig and Xenopus laevis.

Quick Overview for MARCH5 antibody (C-Term) (ABIN6738065)

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Chicken, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCH5 Antibody

Binding Specificity: C-Term

Specificity: Human MARCH5

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MARCH5 (Q9NX47, NP_060294). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish (100%), Trout, Salmon, Stickleback (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MARCH5

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Alternative Name: MARCH5

Background: Name/Gene ID: MARCH5

Synonyms: MARCH5, MARCH-V, RING finger protein 153, RNF153, Mitochondrial ubiquitin ligase, MITOL

Gene ID: 54708

NCBI Accession: NP_060294

UniProt: Q9NX47