EBP antibody (AA 77-126)

Catalog No. ABIN6738267

The Rabbit Polyclonal anti-EBP antibody is suitable to detect EBP in samples from Human, Mouse, Rat and Guinea Pig. It has been validated for WB.

Quick Overview for EBP antibody (AA 77-126) (ABIN6738267)

Target: EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EBP Antibody

Binding Specificity: AA 77-126

Specificity: Human EBP

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Bovine, Pig (92%) Guinea pig (85%) Mouse, Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa77-126 of human EBP (Q15125, NP_006570). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Galago, Elephant (100%), Monkey, Marmoset, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig (92%), Guinea pig (85%), Mouse, Rat, Hamster (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EBP

Target: EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))

Alternative Name: EBP

Background: Name/Gene ID: EBP

Synonyms: EBP, CDPX2, CPXD, CHO2, Cholestenol Delta-isomerase, CPX, D8-D7 sterol isomerase, Emopamil-binding protein, Sterol 8-isomerase

Gene ID: 10682

NCBI Accession: NP_006570

UniProt: Q15125