NCKAP1L antibody (AA 1008-1057)

Catalog No. ABIN6738380

Product Details anti-NCKAP1L Antibody

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Binding Specificity: AA 1008-1057

Reactivity: Human, Dog, Cow, Rabbit, Horse, Guinea Pig, Monkey, Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCKAP1L antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human NCKAP1L

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit, Pig, Guinea pig (100%) Mouse, Rat (92%) Dog (85%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1008-1057 of human NCKAP1L (P55160, NP_005328). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Mouse, Rat, Elephant (92%), Dog (85%), Bovine (84%), Xenopus (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NCKAP1L

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Alternative Name: NCKAP1L (NCKAP1L Products)

Synonyms: HEM1 antibody, 4930568P13Rik antibody, AI463083 antibody, Hem1 antibody, Hemp1 antibody, NCK associated protein 1 like antibody, zgc:172352 antibody, nck-associated protein 1-like antibody, NCK-associated protein 1-like antibody, NCKAP1L antibody, zgc:172352 antibody, LOC100030968 antibody, nckap1l antibody, LOC100618382 antibody, LOC100639088 antibody, Nckap1l antibody

Background: Name/Gene ID: NCKAP1L

Synonyms: NCKAP1L, HEM1, Hematopoietic protein 1, NCK-associated protein 1-like

Gene ID: 3071

NCBI Accession: NP_005328

UniProt: P55160

Pathways: Regulation of Actin Filament Polymerization