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Vimentin antibody (AA 110-159)

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB. It is suitable to detect Vimentin in samples from Human, Mouse, Rat, Pig, Cow, Monkey, Dog, Rabbit, Sheep, Hamster, Horse, Guinea Pig and Bat.
Catalog No. ABIN6738431

Quick Overview for Vimentin antibody (AA 110-159) (ABIN6738431)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 376
  • 129
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Human, Mouse, Rat, Pig, Cow, Monkey, Dog, Rabbit, Sheep, Hamster, Horse, Guinea Pig, Bat

Host

  • 198
  • 164
  • 11
  • 6
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  • 5
  • 2
  • 2
Rabbit

Clonality

  • 203
  • 190
Polyclonal

Conjugate

  • 240
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  • 13
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  • 1
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This Vimentin antibody is un-conjugated

Application

  • 327
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  • 94
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Western Blotting (WB)
  • Binding Specificity

    • 34
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    AA 110-159

    Specificity

    Human VIM / Vimentin

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Sheep (92%) Chicken (85%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa110-159 of human VIM (P08670, NP_003371). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Zebra finch, Chicken, Lizard (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB

    Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin

    Gene ID

    7431

    NCBI Accession

    NP_003371

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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