PYGB antibody (AA 180-229)

Catalog No. ABIN6738478

The Rabbit Polyclonal anti-PYGB antibody has been validated for WB. It is suitable to detect PYGB in samples from Human, Mouse, Rabbit, Sheep, Cow and Monkey.

Quick Overview for PYGB antibody (AA 180-229) (ABIN6738478)

Target: PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))

Reactivity: Human, Mouse, Rabbit, Sheep, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYGB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PYGB Antibody

Binding Specificity: AA 180-229

Specificity: Human PYGB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Rat, Dog, Horse (92%) Rabbit, Chicken, Zebrafish (85%) Sheep, Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human PYGB (P11216, NP_002853). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Platypus (100%), Rat, Dog, Bat, Horse, Opossum, Salmon, Zebrafish (92%), Rabbit, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (85%), Sheep, Bovine (84%), Shrew, Xenopus (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PYGB

Target: PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))

Alternative Name: GPBB / PYGB

Background: Name/Gene ID: PYGB

Synonyms: PYGB, GPBB, Phosphorylase, glycogen, Phosphorylase, glycogen brain, Glycogen phosphorylase B

Gene ID: 5834

NCBI Accession: NP_002853

UniProt: P11216

Pathways: Cellular Glucan Metabolic Process