FLASH antibody (AA 110-159)

Catalog No. ABIN6738722

The Rabbit Polyclonal anti-FLASH antibody has been validated for WB. It is suitable to detect FLASH in samples from Human, Mouse, Rat, Dog and Monkey.

Quick Overview for FLASH antibody (AA 110-159) (ABIN6738722)

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Reactivity: Human, Mouse, Rat, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLASH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FLASH Antibody

Binding Specificity: AA 110-159

Specificity: Human CASP8AP2 / FLASH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Horse, Pig (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa110-159 of human CASP8AP2 (Q9UKL3, NP_036247). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Opossum (100%), Bovine, Horse, Pig (92%), Dog, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FLASH

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Alternative Name: CASP8AP2 / FLASH

Background: Name/Gene ID: CASP8AP2
Family: Apoptosis

Synonyms: CASP8AP2, CASP8 associated protein 2, CASP8-associated protein 2, CED-4, FLICE associated huge, FLICE-associated huge protein, KIAA1315, FLASH, FLASH homolog RIP25, Human FLASH, RIP25, Caspase 8 associated protein 2

Gene ID: 9994

NCBI Accession: NP_036247

UniProt: Q9UKL3

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity