Aspartate beta Hydroxylase antibody (N-Term)

Catalog No. ABIN6738763

The Rabbit Polyclonal anti-Aspartate beta Hydroxylase antibody has been validated for WB. It is suitable to detect Aspartate beta Hydroxylase in samples from Human, Rat, Dog, Cow and Monkey.

Quick Overview for Aspartate beta Hydroxylase antibody (N-Term) (ABIN6738763)

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Reactivity: Human, Rat, Dog, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aspartate beta Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aspartate beta Hydroxylase Antibody

Binding Specificity: N-Term

Specificity: Human ASPH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Mouse (91%) Rat, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human ASPH (Q12797, NP_064549). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog (100%), Elephant, Bat (92%), Mouse (91%), Rat, Bovine (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Aspartate beta Hydroxylase

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Alternative Name: Junctin / ASPH

Background: Name/Gene ID: ASPH

Synonyms: ASPH, A beta H-J-J, AAH, ASP beta-hydroxylase, BAH, Aspartate beta-hydroxylase, CASQ2BP1, Cardiac junctin, HAAH, Humbug, JCTN, Junctate, Junctin

Gene ID: 444

NCBI Accession: NP_064549

UniProt: Q12797

Pathways: Positive Regulation of Endopeptidase Activity