PHGDH antibody (AA 362-411)

Catalog No. ABIN6739000

This anti-PHGDH antibody is a Rabbit Polyclonal antibody detecting PHGDH in WB. Suitable for Human, Mouse, Rat, Hamster, Monkey.

Quick Overview for PHGDH antibody (AA 362-411) (ABIN6739000)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human, Mouse, Rat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHGDH Antibody

Binding Specificity: AA 362-411

Specificity: Human PHGDH

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa362-411 of human PHGDH (O08651, NP_006614). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster (100%), Dog, Rabbit (92%), Galago (90%), Elephant, Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH / PGDH

Background: Name/Gene ID: PHGDH

Synonyms: PHGDH, 3-PGDH, 3PGDH, PGDH, Phosphoglycerate dehydrogenase, PDG, PGAD, PGDH3

Gene ID: 26227

NCBI Accession: NP_006614

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect