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ATG4B antibody (AA 72-121)

This Rabbit Polyclonal antibody specifically detects ATG4B in WB. It exhibits reactivity toward Human, Rat, Guinea Pig, Rabbit, Monkey and Pig.
Catalog No. ABIN6739050

Quick Overview for ATG4B antibody (AA 72-121) (ABIN6739050)

Target

See all ATG4B Antibodies
ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity

  • 107
  • 54
  • 29
  • 8
  • 7
  • 5
  • 5
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Human, Rat, Guinea Pig, Rabbit, Monkey, Pig

Host

  • 104
  • 5
Rabbit

Clonality

  • 101
  • 8
Polyclonal

Conjugate

  • 55
  • 7
  • 7
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ATG4B antibody is un-conjugated

Application

  • 83
  • 36
  • 25
  • 16
  • 14
  • 13
  • 13
  • 11
  • 11
  • 7
  • 3
  • 3
  • 2
Western Blotting (WB)
  • Binding Specificity

    • 20
    • 17
    • 15
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 72-121

    Specificity

    Human ATG4B

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Rat, Guinea pig (100%) Mouse, Dog, Bovine (92%) Rabbit, Pig (84%) Chicken (83%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa72-121 of human ATG4B (Q9Y4P1, NP_037457). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Guinea pig (100%), Mouse, Elephant, Dog, Bovine, Bat (92%), Rabbit, Pig (84%), Chicken (83%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB

    Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

    Alternative Name

    APG4B / ATG4B

    Background

    Name/Gene ID: ATG4B

    Synonyms: ATG4B, APG4 autophagy 4 homolog B, AUTL1, Autophagin-1, Cysteine protease ATG4B, HAPG4B, APG4B, KIAA0943

    Gene ID

    23192

    NCBI Accession

    NP_037457

    UniProt

    Q9Y4P1

    Pathways

    Autophagy
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