Nephronectin antibody (AA 351-400)

Catalog No. ABIN6739216

The Rabbit Polyclonal anti-Nephronectin antibody has been validated for WB. It is suitable to detect Nephronectin in samples from Human, Mouse, Dog, Guinea Pig, Horse and Chicken.

Quick Overview for Nephronectin antibody (AA 351-400) (ABIN6739216)

Target: Nephronectin (NPNT)

Reactivity: Human, Mouse, Dog, Guinea Pig, Horse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nephronectin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nephronectin Antibody

Binding Specificity: AA 351-400

Specificity: Human NPNT / Nephronectin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Horse, Guinea pig, Chicken (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa351-400 of human NPNT (Q6UXI9, NP_001028219). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Panda, Dog, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Orangutan, Marmoset, Elephant, Bovine, Bat, Platypus (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nephronectin

Target: Nephronectin (NPNT)

Alternative Name: NPNT / Nephronectin

Background: Name/Gene ID: NPNT

Synonyms: NPNT, EGFL6L, POEM, Protein EGFL6-like, Nephronectin

Gene ID: 255743

NCBI Accession: NP_001028219

UniProt: Q6UXI9