HELT antibody (AA 141-190)

Catalog No. ABIN6739288

The Rabbit Polyclonal anti-HELT antibody has been validated for WB. It is suitable to detect HELT in samples from Mouse, Human, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat and Monkey.

Quick Overview for HELT antibody (AA 141-190) (ABIN6739288)

Target: HELT (Helt BHLH Transcription Factor (HELT))

Reactivity: Mouse, Human, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HELT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HELT Antibody

Binding Specificity: AA 141-190

Specificity: Human HELT

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Zebrafish (100%) Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa141-190 of human HELT (A6NFD8, NP_001025058). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Panda, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Zebra finch, Lizard, Stickleback, Zebrafish (100%), Elephant, Dog (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HELT

Target: HELT (Helt BHLH Transcription Factor (HELT))

Alternative Name: HELT

Background: Name/Gene ID: HELT

Synonyms: HELT, BHLHb44, Helt bHLH transcription factor, Mgn, HCM1228, Hey-like transcription factor, MEGANE, HESL, Heslike, Megane bHLH factor

Gene ID: 391723

NCBI Accession: NP_001025058

UniProt: A6NFD8

Pathways: Feeding Behaviour