PDPK1 antibody (AA 287-336)

Catalog No. ABIN6740274

The Rabbit Polyclonal anti-PDPK1 antibody is suitable to detect PDPK1 in samples from Human, Mouse, Rat, Monkey, Cow, Dog and Horse. It has been validated for WB.

Quick Overview for PDPK1 antibody (AA 287-336) (ABIN6740274)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 287-336

Specificity: Human PDK1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Bovine, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa287-336 of human PDK1 (Q15118, NP_002601). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Galago, Mouse, Rat, Bovine, Horse, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: Name/Gene ID: PDK1
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK1, PDHK, PDH kinase 1, PDHK1

Gene ID: 5163

NCBI Accession: NP_002601

UniProt: Q15118

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals