ME1 antibody (AA 36-85)

Catalog No. ABIN6740312

The Rabbit Polyclonal anti-ME1 antibody has been validated for WB. It is suitable to detect ME1 in samples from Human, Dog and Pig.

Quick Overview for ME1 antibody (AA 36-85) (ABIN6740312)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ME1 Antibody

Binding Specificity: AA 36-85

Specificity: Human ME1

Predicted Reactivity: Percent identity by BLAST analysis: Sheep, Bovine, Horse (92%) Chicken (90%) Dog, Pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human ME1 (P48163, NP_002386). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%), Monkey, Galago, Marmoset, Sheep, Bovine, Horse (92%), Bat, Opossum, Chicken, Platypus, Lizard (90%), Elephant, Dog, Pig, Stickleback (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: ME1 / Malate Dehydrogenase

Background: Name/Gene ID: ME1

Synonyms: ME1, HUMNDME, Malate dehydrogenase, Malic enzyme 1, Malate oxidoreductase, Malic enzyme 1, soluble, MES, NADP-ME, Pyruvic-malic carboxylase, NADP-dependent malic enzyme, Malic enzyme, cytoplasmic

Gene ID: 4199

NCBI Accession: NP_002386

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha