HIC2 antibody (AA 252-301)
Quick Overview for HIC2 antibody (AA 252-301) (ABIN6740321)
Target
See all HIC2 AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Binding Specificity
- AA 252-301
-
Specificity
- Human HIC2
-
Predicted Reactivity
- Percent identity by BLAST analysis: Human (100%) Mouse, Dog, Bovine (85%).
-
Purification
- Immunoaffinity purified
-
Immunogen
-
Synthetic peptide located between aa252-301 of human HIC2 (Q96JB3, NP_055909). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Gibbon, Monkey, Galago, Rabbit (92%), Mouse, Dog, Bovine, Bat, Horse (85%), Guinea pig (84%), Rat (78%).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
-
-
-
-
Application Notes
-
Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Not recommended for: IHC-P -
Comment
-
Target Species of Antibody: Human
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Distilled water
-
Concentration
- Lot specific
-
Buffer
- Lyophilized from PBS with 2 % sucrose
-
Handling Advice
- Avoid repeat freeze-thaw cycles.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
-
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
-
-
-