PHD1 antibody (AA 281-315)

Catalog No. ABIN6740396

This anti-PHD1 antibody is a Rabbit Polyclonal antibody detecting PHD1 in WB. Suitable for Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig, Monkey and Bat.

Quick Overview for PHD1 antibody (AA 281-315) (ABIN6740396)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHD1 Antibody

Binding Specificity: AA 281-315

Specificity: Human EGLN2 / PHD1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa281-315 of human EGLN2 (Q96KS0). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Stickleback, Pufferfish, Zebrafish (92%), Salmon, Pike (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2 / PHD1

Background: Name/Gene ID: EGLN2

Synonyms: EGLN2, EIT6, EGL nine (C.elegans) homolog 2, Egl nine homolog 2, HPH-1, HPH-3, Estrogen-induced tag 6, PHD1, HIF-PH1, HIF-prolyl hydroxylase 1, HIFPH1

Gene ID: 112398

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis