CHRNA4 antibody (AA 36-85)

Catalog No. ABIN6740407

The Rabbit Polyclonal anti-CHRNA4 antibody has been validated for WB. It is suitable to detect CHRNA4 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Chicken, Monkey and Hamster.

Quick Overview for CHRNA4 antibody (AA 36-85) (ABIN6740407)

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Chicken, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRNA4 Antibody

Binding Specificity: AA 36-85

Specificity: Human CHRNA4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Guinea pig (100%) Rabbit, Zebrafish (92%) Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human CHRNA4 (P43681, NP_000735). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Ferret, Rabbit, Lizard, Zebrafish (92%), Bat (91%), Gibbon, Dog, Bovine, Horse, Pig, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHRNA4

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Alternative Name: CHRNA4 / NACHR

Background: Name/Gene ID: CHRNA4
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNA4, BFNC, AChR-alpha4, Acra-4, EBN, EBN1, NACHR, NAChR alpha 4, NACRA4, NACHRA4, ENFL1

Gene ID: 1137

NCBI Accession: NP_000735

UniProt: P43681

Pathways: Synaptic Membrane