KCNJ1 antibody (AA 216-265)

Catalog No. ABIN6740423

The Rabbit Polyclonal anti-KCNJ1 antibody is suitable to detect KCNJ1 in samples from Human, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Bat and Xenopus laevis. It has been validated for WB.

Quick Overview for KCNJ1 antibody (AA 216-265) (ABIN6740423)

Target: KCNJ1 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNJ1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KCNJ1 Antibody

Binding Specificity: AA 216-265

Specificity: Human KCNJ1 / Kir1.1

Predicted Reactivity: Percent identity by BLAST analysis: Dog, Rabbit, Horse, Guinea pig, Xenopus (100%) Mouse, Rat, Pig, Chicken (92%) Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human KCNJ1 (P48048, NP_000211). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Elephant, Dog, Bat, Rabbit, Horse, Guinea pig, Platypus, Xenopus (100%), Mouse, Rat, Hamster, Dolphin, Pig, Opossum, Turkey, Zebra finch, Chicken (92%), Bovine, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KCNJ1

Target: KCNJ1 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))

Alternative Name: KCNJ1 / ROMK

Background: Name/Gene ID: KCNJ1
Subfamily: Potassium channel - inward-rectifying
Family: Ion channel

Synonyms: KCNJ1, KIR1.1, ROMK1, ROMK3, ROMK5, Inwardly rectifying K+ channel, ROMK, Romk2, ROMK4

Gene ID: 3758

NCBI Accession: NP_000211

UniProt: P48048