Nkx2-2 antibody (N-Term)

Catalog No. ABIN6740533

This Rabbit Polyclonal antibody specifically detects Nkx2-2 in WB. It exhibits reactivity toward Human, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Hamster and Monkey.

Quick Overview for Nkx2-2 antibody (N-Term) (ABIN6740533)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nkx2-2 Antibody

Binding Specificity: N-Term

Specificity: Human NKX2-2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine (100%) Mouse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human NKX2-2 (O95096, NP_002500). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Marmoset, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Guinea pig, Zebra finch, Salmon, Zebrafish (100%), Mouse, Opossum, Lizard (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2-2

Background: Name/Gene ID: NKX2-2

Synonyms: NKX2-2, Homeobox protein Nkx-2.2, NK-2 (Drosophila) homolog B, NK2 homeobox 2, NKX2.2, NKX2B, NK-2 homolog B

Gene ID: 4821

NCBI Accession: NP_002500

UniProt: P42586

Pathways: Dopaminergic Neurogenesis