Notch1 antibody (C-Term)

Catalog No. ABIN6740590

The Rabbit Polyclonal anti-Notch1 antibody has been validated for WB. It is suitable to detect Notch1 in samples from Human, Mouse, Rat, Cow, Dog and Monkey.

Quick Overview for Notch1 antibody (C-Term) (ABIN6740590)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Notch1 Antibody

Binding Specificity: C-Term

Specificity: Human NOTCH1

Predicted Reactivity: Percent identity by BLAST analysis: Horse, Guinea pig, Chicken, Zebrafish (92%) Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NOTCH1 (P46531, NP_060087). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset (100%), Horse, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Zebrafish (92%), Rat, Bat, Xenopus (85%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Background: Name/Gene ID: NOTCH1

Synonyms: NOTCH1, Notch 1, TAN1

Gene ID: 4851

NCBI Accession: NP_060087

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development