CARS antibody (AA 756-805)

Catalog No. ABIN6740660

This Rabbit Polyclonal antibody specifically detects CARS in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Pig and Monkey.

Quick Overview for CARS antibody (AA 756-805) (ABIN6740660)

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Reactivity: Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CARS Antibody

Binding Specificity: AA 756-805

Specificity: Human CARS

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat (100%) Dog, Bovine, Guinea pig, Chicken (92%) Mouse (85%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa756-805 of human CARS (Q5HYE4, NP_001014437). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Rat (100%), Galago, Dog, Bovine, Horse, Guinea pig, Chicken, Lizard (92%), Turkey (91%), Slime mold (90%), Mouse, Drosophila (85%), Salmon, Zebrafish (84%), Xenopus, Stickleback (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CARS

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Alternative Name: CARS1 / CARS

Background: Name/Gene ID: CARS

Synonyms: CARS, Cysteinyl-tRNA synthetase, CYSRS, Cysteine translase, CARS1, MGC:11246

Gene ID: 833

NCBI Accession: NP_001014437

UniProt: P49589