CKM antibody (C-Term)

Catalog No. ABIN6740732

The Rabbit Polyclonal anti-CKM antibody has been validated for WB. It is suitable to detect CKM in samples from Human, Mouse, Rat, Pig, Dog, Rabbit, Cow, Horse, Guinea Pig, Monkey and Bat.

Quick Overview for CKM antibody (C-Term) (ABIN6740732)

Target: CKM (Creatine Kinase, Muscle (CKM))

Reactivity: Human, Mouse, Rat, Pig, Dog, Rabbit, Cow, Horse, Guinea Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CKM Antibody

Binding Specificity: C-Term

Specificity: Human CKM

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rabbit, Pig (100%) Zebrafish (92%) Rat, Dog, Horse, Guinea pig, Chicken (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CKM (P06732, NP_001815). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Platypus (100%), Catfish, Zebrafish (92%), Chicken, Salmon, Smelt (85%), Baboon, Shrew, Hamster, Opossum, Zebra finch, Armadillo (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Alternative Name: CKM / Creatine Kinase MM

Background: Name/Gene ID: CKM

Synonyms: CKM, CK-MB, CKMM, Creatine kinase M-type, Creatine kinase, muscle, M-CK, Creatine kinase M chain

Gene ID: 1158

NCBI Accession: NP_001815

UniProt: P06732