Mesothelin antibody (AA 540-589)

Catalog No. ABIN6740756

This anti-Mesothelin antibody is a Rabbit Polyclonal antibody detecting Mesothelin in WB. Suitable for Human, Mouse, Cow, Guinea Pig and Pig.

Quick Overview for Mesothelin antibody (AA 540-589) (ABIN6740756)

Target: Mesothelin (MSLN)

Reactivity: Human, Mouse, Cow, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Mesothelin Antibody

Binding Specificity: AA 540-589

Specificity: Human MSLN / Mesothelin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Rat, Dog, Horse (92%) Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa540-589 of human MSLN (Q13421-3, NP_005814). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Pig (100%), Monkey, Galago, Marmoset, Rat, Elephant, Panda, Dog, Bat, Horse (92%), Bovine (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN / Mesothelin

Background: Name/Gene ID: MSLN

Synonyms: MSLN, CAK1 antigen, MPF, SMRP, Mesothelin, CAK1

Gene ID: 10232

NCBI Accession: NP_005814

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process