ANO1 antibody (AA 828-877)

Catalog No. ABIN6740769

This Rabbit Polyclonal antibody specifically detects ANO1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig and Horse.

Quick Overview for ANO1 antibody (AA 828-877) (ABIN6740769)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ANO1 Antibody

Binding Specificity: AA 828-877

Specificity: Human ANO1 / TMEM16A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa828-877 of human ANO1 (Q5XXA6, NP_060513). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Horse, Guinea pig (100%), Marmoset, Zebra finch (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: ANO1 / DOG1 / TMEM16A

Background: Name/Gene ID: ANO1

Synonyms: ANO1, Oral cancer overexpressed 2, Transmembrane protein 16A, ORAOV2, Anoctamin-1, DOG1, TAOS2, TMEM16A

Gene ID: 55107

NCBI Accession: NP_060513

UniProt: Q5XXA6