VARS antibody (C-Term)

Catalog No. ABIN6740955

The Rabbit Polyclonal anti-VARS antibody has been validated for WB. It is suitable to detect VARS in samples from Human, Mouse, Cow, Horse, Rabbit, Dog, Pig and Monkey.

Quick Overview for VARS antibody (C-Term) (ABIN6740955)

Target: VARS (Valyl-tRNA Synthetase (VARS))

Reactivity: Human, Mouse, Cow, Horse, Rabbit, Dog, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VARS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VARS Antibody

Binding Specificity: C-Term

Specificity: Human VARS

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine, Rabbit, Horse, Pig (100%) Rat, Guinea pig (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human VARS (P26640, NP_006286). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Bovine, Rabbit, Horse, Pig (100%), Galago, Rat, Elephant, Panda, Guinea pig (92%), Dog, Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for VARS

Target: VARS (Valyl-tRNA Synthetase (VARS))

Alternative Name: VARS / ValRS

Background: Name/Gene ID: VARS

Synonyms: VARS, G7a, Valyl-tRNA synthetase 2, Protein G7a, Valine--tRNA ligase, ValRS, Valyl-tRNA synthetase, VARS1

Gene ID: 7407

NCBI Accession: NP_006286

UniProt: P26640