AFG3L2 antibody (AA 324-373)

Catalog No. ABIN6740990

This Rabbit Polyclonal antibody specifically detects AFG3L2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig and Xenopus laevis.

Quick Overview for AFG3L2 antibody (AA 324-373) (ABIN6740990)

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFG3L2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AFG3L2 Antibody

Binding Specificity: AA 324-373

Specificity: Human AFG3L2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa324-373 of human AFG3L2 (Q9Y4W6, NP_006787). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Turkey, Chicken, Stickleback (92%), Drosophila, Arabidopsis (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AFG3L2

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Alternative Name: AFG3L2

Background: Name/Gene ID: AFG3L2
Subfamily: Metallopeptidase M41
Family: Protease

Synonyms: AFG3L2, AFG3-like protein 2, SPAX5, Spinocerebellar ataxia 28, Paraplegin-like protein, ATPase family gene 3, yeast, SCA28

Gene ID: 10939

NCBI Accession: NP_006787

UniProt: Q9Y4W6

Pathways: Skeletal Muscle Fiber Development