EIF3E antibody (AA 360-409)

Catalog No. ABIN6741024

The Rabbit Polyclonal anti-EIF3E antibody (ABIN6741024) specifically detects EIF3E in WB. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig, Bat, Chicken and Xenopus laevis samples.

Quick Overview for EIF3E antibody (AA 360-409) (ABIN6741024)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig, Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF3E Antibody

Binding Specificity: AA 360-409

Specificity: Human EIF3E

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa360-409 of human EIF3E (P60228, NP_001559). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Trout, Catfish, Salmon, Smelt, Sablefish, Zebrafish (100%), Stickleback (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E

Background: Name/Gene ID: EIF3E

Synonyms: EIF3E, EIF3-p46, INT6, EIF-3 p48, EIF3-P48, EIF3S6

Gene ID: 3646

NCBI Accession: NP_001559

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C