SPTLC2 antibody (AA 72-121)

Catalog No. ABIN6741036

The Rabbit Polyclonal anti-SPTLC2 antibody has been validated for WB. It is suitable to detect SPTLC2 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Hamster, Monkey and Xenopus laevis.

Quick Overview for SPTLC2 antibody (AA 72-121) (ABIN6741036)

Target: SPTLC2 (serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Hamster, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTLC2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPTLC2 Antibody

Binding Specificity: AA 72-121

Specificity: Human SPTLC2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Guinea pig, Zebrafish (100%) Horse (92%) Xenopus (90%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human SPTLC2 (O15270, NP_004854). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Opossum, Guinea pig, Platypus, Xenopus, Zebrafish (100%), Bat, Horse (92%), Panda, Dog, Pig, Zebra finch, Stickleback (90%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPTLC2

Target: SPTLC2 (serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))

Alternative Name: SPTLC2 / LCB2

Background: Name/Gene ID: SPTLC2

Synonyms: SPTLC2, HSN1C, HLCB2a, KIAA0526, LCB2, LCB2A, SPT 2, NSAN1C, SPT2, LCB 2, Serine palmitoyltransferase 2

Gene ID: 9517

NCBI Accession: NP_004854

UniProt: O15270