POLR2K antibody (N-Term)

Catalog No. ABIN6741037

This anti-POLR2K antibody is a Rabbit Polyclonal antibody detecting POLR2K in WB. Suitable for Human, Mouse, Rat, Cow, Monkey, Guinea Pig, Horse, Rabbit, Hamster and Pig.

Quick Overview for POLR2K antibody (N-Term) (ABIN6741037)

Target: POLR2K (Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Guinea Pig, Horse, Rabbit, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2K antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2K Antibody

Binding Specificity: N-Term

Specificity: Human POLR2K

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rabbit, Guinea pig (100%) Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human POLR2K (P53803, NP_005025). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Zebra finch, Chicken, Xenopus, Salmon, Smelt, Sablefish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR2K

Target: POLR2K (Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K))

Alternative Name: POLR2K

Background: Name/Gene ID: POLR2K

Synonyms: POLR2K, ABC10-alpha, HsRPB10a, HRPB7.0, RPB12, RPB7.0, RPABC4, RPB10alpha

Gene ID: 5440

NCBI Accession: NP_005025

UniProt: P53803

Pathways: Regulatory RNA Pathways