Gephyrin antibody (AA 396-445)

Catalog No. ABIN6741118

This Rabbit Polyclonal antibody specifically detects Gephyrin in WB. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Monkey, Cow, Dog, Guinea Pig, Horse, Rabbit, Xenopus laevis, Bat and Pig.

Quick Overview for Gephyrin antibody (AA 396-445) (ABIN6741118)

Target: Gephyrin (GPHN)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Monkey, Cow, Dog, Guinea Pig, Horse, Rabbit, Xenopus laevis, Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gephyrin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Gephyrin Antibody

Binding Specificity: AA 396-445

Specificity: Human GPHN / Gephyrin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human GPHN (Q9NQX3, NP_001019389). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Gephyrin

Target: Gephyrin (GPHN)

Alternative Name: Gephyrin

Background: Name/Gene ID: GPHN

Synonyms: GPHN, Gephyrin, KIAA1385, GEPH, GPH, GPHRYN, HKPX1, MOCODC

Gene ID: 10243

NCBI Accession: NP_001019389

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development