CHCHD4 antibody (N-Term)

Catalog No. ABIN6741119

The Rabbit Polyclonal anti-CHCHD4 antibody is suitable to detect CHCHD4 in samples from Human, Mouse, Rat, Guinea Pig, Xenopus laevis and Chicken. It has been validated for WB.

Quick Overview for CHCHD4 antibody (N-Term) (ABIN6741119)

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Reactivity: Human, Mouse, Rat, Guinea Pig, Xenopus laevis, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHCHD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHCHD4 Antibody

Binding Specificity: N-Term

Specificity: Human CHCHD4 / MIA40

Predicted Reactivity: Percent identity by BLAST analysis: Guinea pig, Chicken (100%) Dog, Rabbit, Horse, Pig (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human CHCHD4 (Q8N4Q1, NP_001091972). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus (100%), Monkey, Marmoset, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Seabass (92%), Zebrafish (90%), Salmon, Sablefish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHCHD4

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Alternative Name: MIA40 / CHCHD4

Background: Name/Gene ID: CHCHD4

Synonyms: CHCHD4, CHDH4, TIMM40, MIA40

Gene ID: 131474

NCBI Accession: NP_001091972

UniProt: Q8N4Q1