CAPN10 antibody (N-Term)

Catalog No. ABIN6741135

The Rabbit Polyclonal anti-CAPN10 antibody has been validated for WB. It is suitable to detect CAPN10 in samples from Human, Dog, Rabbit, Bat and Monkey.

Quick Overview for CAPN10 antibody (N-Term) (ABIN6741135)

Target: CAPN10 (Calpain 10 (CAPN10))

Reactivity: Human, Dog, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAPN10 Antibody

Binding Specificity: N-Term

Specificity: Human CAPN10 / Calpain 10

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit (100%) Mouse, Rat, Guinea pig (92%) Bovine (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human CAPN10 (Q9HC96, NP_075573). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Dog, Bat, Rabbit (100%), Mouse, Rat, Guinea pig (92%), Bovine (91%), Opossum (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CAPN10

Target: CAPN10 (Calpain 10 (CAPN10))

Alternative Name: CAPN10 / Calpain 10

Background: Name/Gene ID: CAPN10
Subfamily: Cysteine C2
Family: Protease

Synonyms: CAPN10, Calpain-10, Calpain-like protease CAPN10, CANP10, KIAA1845, NIDDM1, Calpain 10, CANP 10

Gene ID: 11132

NCBI Accession: NP_075573

UniProt: Q9HC96

Pathways: Positive Regulation of Peptide Hormone Secretion