CAPN10 antibody (N-Term)

Catalog No. ABIN7219246

This Rabbit Polyclonal antibody specifically detects CAPN10 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CAPN10 antibody (N-Term) (ABIN7219246)

Target: CAPN10 (Calpain 10 (CAPN10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CAPN10 Antibody

Binding Specificity: N-Term

Purpose: Rabbit Anti-Calpain 10 Polyclonal Antibody

Specificity: Calpain 10 Polyclonal Antibody detects endogenous levels of Calpain 10 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Calpain 10

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CAPN10

Target: CAPN10 (Calpain 10 (CAPN10))

Alternative Name: Calpain 10

Background: CAPN10, KIAA1845, Calpain-10, Calcium-activated neutral proteinase 10, CANP 10Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation, domain II, the protease domain, domain III, a linker domain of unknown function, and domain IV, the calmodulin-like calcium-binding domain. CAPN10 encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. CAPN10 is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene.

Molecular Weight: 75kD

Gene ID: 11132

UniProt: Q9HC96

Pathways: Positive Regulation of Peptide Hormone Secretion