Septin 12 antibody (AA 180-229)

Catalog No. ABIN6741160

The Rabbit Polyclonal anti-Septin 12 antibody has been validated for WB. It is suitable to detect Septin 12 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Pig and Monkey.

Quick Overview for Septin 12 antibody (AA 180-229) (ABIN6741160)

Target: Septin 12 (Sep12)

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Septin 12 Antibody

Binding Specificity: AA 180-229

Specificity: Human SEPT12 / Septin 12

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Dog, Bovine (92%) Salmon, Pufferfish, Zebrafish (90%) Xenopus (84%) Neurospora, Trichoplax, Tunicate, Paracoccidioides (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human SEPT12 (Q8IYM1, NP_653206). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Gibbon, Dog, Opossum (92%), Platypus (91%), Turkey, Salmon, Stickleback, Zebrafish (90%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Septin 12

Target: Septin 12 (Sep12)

Alternative Name: SEPT12 / Septin 12

Background: Name/Gene ID: SEPT12

Synonyms: SEPT12, Septin-12, Septin 12, SPGF10

Gene ID: 124404

NCBI Accession: NP_653206

UniProt: Q8IYM1