ADHFE1 antibody (AA 288-337)

Catalog No. ABIN6741163

The Rabbit Polyclonal anti-ADHFE1 antibody has been validated for WB. It is suitable to detect ADHFE1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Bat, Monkey and Xenopus laevis.

Quick Overview for ADHFE1 antibody (AA 288-337) (ABIN6741163)

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Bat, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADHFE1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADHFE1 Antibody

Binding Specificity: AA 288-337

Specificity: Human ADHFE1

Predicted Reactivity: Percent identity by BLAST analysis: Dog, Horse, Guinea pig (100%) Rabbit (92%) Chicken (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa288-337 of human ADHFE1 (Q8IWW8, NP_653251). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Guinea pig, Xenopus (100%), Galago, Bovine, Rabbit, Pig, Opossum (92%), Platypus (91%), Turkey, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ADHFE1

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Alternative Name: ADHFE1

Background: Name/Gene ID: ADHFE1

Synonyms: ADHFE1, ADH8, Alcohol dehydrogenase 8, HMFT2263, HOT

Gene ID: 137872

NCBI Accession: NP_653251

UniProt: Q8IWW8