RHOC antibody (AA 35-84)

Catalog No. ABIN6741373

The Rabbit Polyclonal anti-RHOC antibody (ABIN6741373) specifically detects RHOC in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio), Drosophila melanogaster, Chicken, Insect, Monkey, Pig and Xenopus laevis samples.

Quick Overview for RHOC antibody (AA 35-84) (ABIN6741373)

Target: RHOC (Ras Homolog Gene Family, Member C (RHOC))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio), Drosophila melanogaster, Chicken, Insect, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RHOC Antibody

Binding Specificity: AA 35-84

Specificity: Human RHOC

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human RHOC (P08134, NP_786886). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Seabass, Catfish, Smelt, Sablefish, Stickleback, Pike, Zebrafish, Drosophila, Mosquito, Slime mold, Nematode (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RHOC

Target: RHOC (Ras Homolog Gene Family, Member C (RHOC))

Alternative Name: RHOC

Background: Name/Gene ID: RHOC

Synonyms: RHOC, ARHC, Rho cDNA clone 9, RhoC GTPase, RHOH9, Small GTP binding protein RhoC, ARH9, H9, Oncogene RHO H9, Ras homolog family member C, RAS-related homolog 9

Gene ID: 389

NCBI Accession: NP_786886

UniProt: P08134

Pathways: WNT Signaling, Cell-Cell Junction Organization