PSMB3 antibody (AA 71-120)

Catalog No. ABIN6741388

This anti-PSMB3 antibody is a Rabbit Polyclonal antibody detecting PSMB3 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Bat and Monkey.

Quick Overview for PSMB3 antibody (AA 71-120) (ABIN6741388)

Target: PSMB3 (Proteasome Subunit beta 3 (PSMB3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMB3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMB3 Antibody

Binding Specificity: AA 71-120

Specificity: Human PSMB3

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit (100%) Guinea pig, Chicken (92%) Xenopus, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human PSMB3 (P49720, NP_002786). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Arabidopsis (100%), Guinea pig, Turkey, Chicken, Lizard, Smelt, Stickleback (92%), Drosophila, Rice, Grape (90%), Zebra finch, Xenopus, Trout, Catfish, Salmon, Zebrafish, Sea squirt, Slime mold (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PSMB3

Target: PSMB3 (Proteasome Subunit beta 3 (PSMB3))

Alternative Name: PSMB3

Background: Name/Gene ID: PSMB3
Subfamily: Threonine T1
Family: Protease Non Catalytic

Synonyms: PSMB3, HC10-II, Macropain theta chain, Proteasome chain 13, Proteasome theta chain, Proteasome subunit beta type-3, C10-ii, Proteasome component C10-II

Gene ID: 5691

NCBI Accession: NP_002786

UniProt: P49720

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Cell RedoxHomeostasis, Lipid Metabolism