FOSL1 antibody (AA 130-179)

Catalog No. ABIN462165

This anti-FOSL1 antibody is a Rabbit Polyclonal antibody detecting FOSL1 in WB, IHC and IHC (p). Suitable for Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat and Monkey.

Quick Overview for FOSL1 antibody (AA 130-179) (ABIN462165)

Target: FOSL1 (FOS-Like Antigen 1 (FOSL1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOSL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOSL1 Antibody

Binding Specificity: AA 130-179

Brand: IHC-plus™

Specificity: Human FOSL1 / FRA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine (100%) Rabbit, Pig (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa130-179 of human FOSL1 (P15407, NP_005429). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Horse, Guinea pig (100%), Rabbit, Pig, Platypus (92%), Sheep, Hamster, Panda, Cat, Opossum, Xenopus, Stickleback, Pufferfish, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOSL1

Target: FOSL1 (FOS-Like Antigen 1 (FOSL1))

Alternative Name: FRA-1 / FOSL1

Background: Name/Gene ID: FOSL1

Synonyms: FOSL1, FOS-like antigen 1, Fos-related antigen 1, FRA, FOS-like antigen-1, FRA1, Fra-1

Gene ID: 8061

NCBI Accession: NP_005429

UniProt: P15407