PAX4 antibody (AA 166-215)

Catalog No. ABIN462174

This anti-PAX4 antibody is a Rabbit Polyclonal antibody detecting PAX4 in WB, IHC and IHC (p). Suitable for Human.

Quick Overview for PAX4 antibody (AA 166-215) (ABIN462174)

Target: PAX4 (Paired Box 4 (PAX4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAX4 Antibody

Binding Specificity: AA 166-215

Brand: IHC-plus™

Specificity: Human PAX4

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Bovine, Rabbit, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa166-215 of human PAX4 (A4D0Z1, NP_006184). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey (100%), Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PAX4

Target: PAX4 (Paired Box 4 (PAX4))

Alternative Name: PAX4

Background: Name/Gene ID: PAX4

Synonyms: PAX4, KPD, Paired box protein Pax-4, Paired domain gene 4, Paired box 4, Paired box gene 4, MODY9

Gene ID: 5078

NCBI Accession: NP_006184

UniProt: O43316