PAX4 antibody (Internal Region)

Catalog No. ABIN6259146

The Rabbit Polyclonal anti-PAX4 antibody is suitable to detect PAX4 in samples from Human, Mouse and Rat. It has been validated for WB, IF and ICC.

Quick Overview for PAX4 antibody (Internal Region) (ABIN6259146)

Target: PAX4 (Paired Box 4 (PAX4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PAX4 Antibody

Binding Specificity: Internal Region

Purpose: PAX4 Antibody

Specificity: PAX4 Antibody detects endogenous levels of total PAX4.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PAX4, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PAX4

Target: PAX4 (Paired Box 4 (PAX4))

Alternative Name: PAX4

Background:

Description: Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.

Gene: PAX4

Molecular Weight: 42 kDa

Gene ID: 5078

UniProt: O43316