PAX7 antibody (AA 167-216)

Catalog No. ABIN462176

The Rabbit Polyclonal anti-PAX7 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PAX7 in samples from Human, Mouse, Rat, Chicken, Dog, Guinea Pig, Cow, Rabbit, Bat and Monkey.

Quick Overview for PAX7 antibody (AA 167-216) (ABIN462176)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human, Mouse, Rat, Chicken, Dog, Guinea Pig, Cow, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAX7 Antibody

Binding Specificity: AA 167-216

Brand: IHC-plus™

Specificity: Human PAX7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken (100%) Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa167-216 of human PAX7 (P23759, NP_002575). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken (100%), Xenopus, Salmon, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.05 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background: Name/Gene ID: PAX7

Synonyms: PAX7, HUP1, Paired box 7, Paired box gene 7, Paired box homeotic gene 7, Paired box protein Pax-7, RMS2, Paired domain gene 7, PAX7 transcriptional factor, PAX7B

Gene ID: 5081

NCBI Accession: NP_002575

UniProt: P23759