PAX7 antibody (AA 301-505)

Catalog No. ABIN6940260

The Mouse Monoclonal anti-PAX7 antibody is suitable to detect PAX7 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio) and Chicken. It has been validated for WB, FACS and IF.

Quick Overview for PAX7 antibody (AA 301-505) (ABIN6940260)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PAX7-497

Product Details anti-PAX7 Antibody

Binding Specificity: AA 301-505

Purpose: PAX7 (Rhabdomyosarcoma Marker) Antibody

Specificity: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (aa301-505) of human PAX7 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HeLa, Jurkat, A431, MCF-7, U2OS or NIH/3T3 cells. Rhabdomyosarcoma (RMS).

Known Application: Immunofluorescence (0.5-1 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background:

HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2

A431, HeLa, Jurkat, MCF-7, U2OS or NIH/3T3 cells. Rhabdomyosarcoma (RMS).

Molecular Weight: 57kDa

Gene ID: 5081, 113253

UniProt: P23759