NRF1 antibody (AA 360-409)

Catalog No. ABIN462197

The Rabbit Polyclonal anti-NRF1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NRF1 in samples from Human, Rat, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio), Dog, Monkey, Chicken, Pig and Xenopus laevis.

Quick Overview for NRF1 antibody (AA 360-409) (ABIN462197)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human, Rat, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio), Dog, Monkey, Chicken, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NRF1 Antibody

Binding Specificity: AA 360-409

Brand: IHC-plus™

Specificity: Human NRF1

No Cross-Reactivity: Mouse (Murine)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1 / NRF-1

Background: Name/Gene ID: NRF1
Family: Transcription factor

Synonyms: NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL

Gene ID: 4899

NCBI Accession: NP_005002

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha