FUBP1 antibody (AA 540-589)

Catalog No. ABIN6741561

This Rabbit Polyclonal antibody specifically detects FUBP1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Cow, Rabbit, Pig and Monkey.

Quick Overview for FUBP1 antibody (AA 540-589) (ABIN6741561)

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Reactivity: Human, Rat, Cow, Rabbit, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FUBP1 Antibody

Binding Specificity: AA 540-589

Brand: IHC-plus™

Specificity: Human FUBP1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Bovine, Rabbit (100%) Dog (93%) Mouse, Guinea pig (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa540-589 of human FUBP1 (Q96AE4, NP_003893). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Rat, Elephant, Bovine, Rabbit, Pig (100%), Gorilla, Dog (93%), Mouse, Guinea pig, Stickleback (92%), Galago (87%), Turkey, Chicken, Platypus (85%), Bat (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FUBP1

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Alternative Name: FUBP1

Background: Name/Gene ID: FUBP1

Synonyms: FUBP1, DNA helicase V, FBP, HDH V, FUSE-binding protein 1, FUBP

Gene ID: 8880

NCBI Accession: NP_003893

UniProt: Q96AE4