MAZ antibody (N-Term)

Catalog No. ABIN6741567

This Rabbit Polyclonal antibody specifically detects MAZ in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Hamster.

Quick Overview for MAZ antibody (N-Term) (ABIN6741567)

Target: MAZ (MYC-Associated Zinc Finger Protein (Purine-Binding Transcription Factor) (MAZ))

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAZ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAZ Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human MAZ

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human MAZ (P56270, NP_002374). Percent identity by BLAST analysis: Human, Chimpanzee, Mouse, Rat, Hamster (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAZ

Target: MAZ (MYC-Associated Zinc Finger Protein (Purine-Binding Transcription Factor) (MAZ))

Alternative Name: MAZ

Background: Name/Gene ID: MAZ

Synonyms: MAZ, Pur-1, SAF-2, SAF-3, MAZI, ZF87, Zif87, ZNF801, Transcription factor Zif87, Zinc finger protein 801, PUR1, SAF-1

Gene ID: 4150

NCBI Accession: NP_002374

UniProt: P56270

Pathways: Chromatin Binding